Figure 4. vPARP protects against cell death.
Human bronchial smooth muscle cells were transfected with control siRNA or siRNA to knockdown vPARP (Santa Cruz). (A) The cell number was determined by counting on a hemocytometer. (B & C) Cell lysates were subjected to immunoblotting to monitor the expression of cleaved caspase-3 (Cell Signaling Technology, Danvers, MA, USA) and vPARP (Santa Cruz). Bar graphs represent means ± SEM (n = 4). * denotes values significantly different from control at P<0.05.
