Fig 1. EA and UA inhibit MPO and LPO activity in dose dependent manner: EA or UA (0–100 μM) was pre-incubated at the indicated final concentrations with MPO (12.5 μg/ml) or LPO (25 μg/ml) for 5 min in 20 μl reaction volume before addition of 30 μl of H2O2 (6.7x10-3%) and 50 μl of 100 mmol/L guaiacol.
Bar graph represent EA and UA mediated dose-dependent inhibition of MPO (A) and LPO (B) activity. Line graph represents EA (50 μM) and UA (50 μM) mediated inhibition of MPO (C) and LPO (D) activity with time. Assays were carried out in 96-well plates in triplicates with appropriate vehicles/buffers. Results are representative of three independent experiments and are expressed as mean ± SEM. *p < 0.05.