Table 1. Ingredients, chemical composition and fatty acid composition of experimental diets.
| Item2 | Treatment1 | ||
|---|---|---|---|
| C | L | L-A | |
| Ingredient, % DM | |||
| Barley | 74.03 | 65.17 | 66.19 |
| Soybean meal | 22.37 | 16.94 | 19.20 |
| Extruded linseed (Valomega 160)3 | - | 14.29 | 7.15 |
| Marine microalgae (DHA-Gold)4 | - | - | 3.89 |
| CaCO3 | 1.76 | 1.76 | 1.76 |
| NaHCO3 | 1.00 | 1.00 | 1.00 |
| NaCl | 0.50 | 0.50 | 0.50 |
| Mineral vitamin supplement5 | 0.30 | 0.30 | 0.30 |
| Flavorings | 0.04 | 0.04 | 0.04 |
| Chemical composition, % DM | |||
| CP | 16.87 | 16.63 | 16.54 |
| Crude fat | 2.93 | 6.10 | 6.68 |
| Crude fiber | 4.35 | 4.63 | 4.30 |
| Ash | 5.23 | 5.54 | 5.77 |
| Metabolizable energy, Mcal/kg | 2.70 | 2.75 | 2.80 |
| Fatty acid composition, g/100 g of total FAME | |||
| C16:0 | 24.0 | 13.2 | 19.9 |
| C18:0 | 3.29 | 3.20 | 2.60 |
| C20:0 | 0.11 | 0.13 | 0.15 |
| C18:1 c-9 | 12.1 | 15.8 | 11.1 |
| C18:2 c-9, t-11 (CLA) | 0.24 | 0.25 | 0.08 |
| C18:2 n-6 (LA) | 48.5 | 30.5 | 28.3 |
| C18:3 n-6 | 0.05 | 0.17 | 0.16 |
| C18:3 n-3 (ALA) | 4.43 | 33.5 | 22.6 |
| C20:5 n-3 (EPA) | 0.08 | 0.04 | 0.34 |
| C22:6 n-3 (DHA) | 0.01 | 0.01 | 6.61 |
1Treatments: C = control; L = 10% linseed; L-A = 5% linseed with 3.89% marine algae.
2CP = crude protein; FAME = fatty acid methyl esters; LA = linoleic acid; ALA = α-linolenic acid; EPA = eicosapentaenoic acid; DHA = docosahexaenoic acid.
3Valomega 160 (Pinallet S.A., Cardona, Spain). Product consisted of 70% Tradi-LIN extruded linseed and 30% wheat bran.
4DHA-Gold (Market Biosciences Corp., Columbia, MD, USA). Algal meal high in DHA derived from Schizochytrium spp.
5Composition, content per kilogram: vitamin A (2,000,000 IU), vitamin D3 (550,000 IU), vitamin E (2000 mg), vitamin B2 (125 mg), vitamin B1 (125 mg), Mg (12,500 mg), Mn (6125 mg), Zn (9900 mg), I (100 mg), Fe (3300 mg), Cu (1100 mg), Co (150 mg), Se (25 mg), anti-rust (62.5 mg).