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. 2016 Jun 2;11(6):e0156765. doi: 10.1371/journal.pone.0156765

Table 4. Effects of addition of linseed or linseed and marine algae to the diet on fatty acid composition (g/100 g of total identified fatty acid methyl esters) in subcutaneous adipose tissue of Navarra breed lambs.

Item2 Treatment1
C L L-A SEM P-value
SFA
    C12:0 0.86Aa 0.51Bb 0.71a 0.05 <0.001
    C14:0 6.31A 5.05B 6.35A 0.22 <0.001
    C16:0 27.5C 26.0B 32.0A 0.38 <0.001
    C18:0 11.9ab 12.8A 11.5B 0.30 0.028
    C20:0 0.09a 0.08b 0.08b 0.00 0.030
    Total SFA3 49.9B 49.9B 54.8A 0.80 <0.001
MUFA
    C16:1 c-9 1.86A 1.34B 1.37B 0.07 <0.001
    C18:1 t-10 + C18:1 t-11 6.66C 13.41A 11.53B 0.50 <0.001
    C18:1 c-9 (oleic acid) 28.4A 22.2B 21.4B 0.60 <0.001
    C18:1 c-11 1.73A 1.13B 1.27B 0.05 <0.001
    C20:1 c-11 0.04b 0.05Aa 0.03Bb 0.00 0.001
    Total MUFA3 42.2Aa 41.0a 38.1Bb 0.70 0.001
C18:2 c-9, t-11 (CLA) 0.16A 0.15A 0.11B 0.01 <0.001
∑CLA4 0.40B 0.48A 0.31C 0.02 <0.001
n-3 PUFA
    C18:3 n-3 (ALA) 0.37C 1.92A 0.84B 0.05 <0.001
    C20:5 n-3 (EPA) 0.07B 0.10B 0.21A 0.01 <0.001
    C22:5 n-3 (DPA) 0.05C 0.08B 0.12A 0.01 <0.001
    C22:6 n-3 (DHA) 0.02B 0.04B 0.58A 0.03 <0.001
    Total n-3 PUFA 0.51C 2.14A 1.75B 0.05 <0.001
n-6 PUFA
    C18:2 n-6 t-9, t-12 0.10C 1.18A 0.50B 0.04 <0.001
    C18:2 n-6 (LA) 3.93A 2.46Bc 1.89Bd 0.14 <0.001
    C20:3 n-6 0.04B 0.05B 0.08A 0.05 <0.001
    C20:4 n-6 (AA) 0.17B 0.15B 0.28A 0.01 <0.001
    Total n-6 PUFA3 4.24a 3.84ab 3.25a 0.19 0.035
Total PUFA 4.57B 5.84A 4.51B 0.23 <0.001
n-6 PUFA/n-3 PUFA 8.32A 1.78B 2.82C 0.10 <0.001
PUFA/SFA 0.10AB 0.12A 0.08B 0.01 0.006

a,b,cMeans with different lowercase supercripts within a row are different (P < 0.05).

A,B,CMeans with different uppercase supercripts within a row are different (P < 0.01).

1Treatments: C = control; L = 10% linseed; L-A = 5% linseed with 3.89% marine algae.

2ALA = α-linolenic acid; EPA = eicosapentaenoic acid; DPA = docosapentaenoic acid; DHA = docosahexaenoic acid; LA = linoleic acid; AA = arachidonic acid.

3Total SFA, MUFA and n-6 PUFA is the sum of all SFA, MUFA and n-6 PUFA.

4∑CLA = C18:2 c-9, t-11 + C18:2 t-10, c-12 + C18:2 c-9, c-11 + C18:2 t-9, t-11.