. 2016 Jun 2;11(6):e0156765. doi: 10.1371/journal.pone.0156765

Table 6. Effects of addition of linseed or linseed and marine algae to the diet on relative mRNA expression of lipid metabolism genes in subcutaneous and intramuscular adipose tissues of Navarra breed lambs.

Gene¹	Tissue	Treatment²	FC³	Std. Error⁴	P-value	Result
PPARG	SC	L	7.42	2.99–17.29	0.012	UP
		L-A	10.6	1.57–50.19	0.032	UP
	IM	L	0.98	0.38–2.61	0.961	NR⁵
		L-A	0.87	0.27–2.39	0.771	NR
CEBPA	SC	L	1.94	1.29–2.80	0.024	UP
		L-A	8.13	1.48–23.80	0.040	UP
	IM	L	0.54	0.27–1.15	0.119	NR
		L-A	0.54	0.27–1.15	0.099	NR
SREBF1	SC	L	1.01	0.60–1.92	0.977	NR
		L-A	1.70	0.18–6.12	0.518	NR
	IM	L	1.12	0.43–3.11	0.813	NR
		L-A	0.44	0.14–1.18	0.110	NR
SCD	SC	L	0.83	0.49–1.43	0.213	NR
		L-A	0.51	0.37–0.88	0.003	DOWN
	IM	C/L	0.07	0.04–0.13	<0.001	DOWN
		L-A	0.53	0.29–0.95	<0.001	DOWN
ACACA	SC	L	0.74	0.53–1.25	0.044	DOWN
		L-A	0.09	0.05–0.13	<0.001	DOWN
	IM	L	0.61	0.37–0.98	<0.001	DOWN
		L-A	0.24	0.15–0.41	<0.001	DOWN
LPL	SC	L	1.18	0.73–1.77	0.169	NR
		L-A	0.52	0.34–0.78	0.002	DOWN
	IM	L	1.83	1.09–3.16	0.095	NR
		L-A	0.41	0.23–0.73	0.032	DOWN
FASD1	SC	L	2.13	0.49–16.33	0.609	NR
		L-A	0.26	0.08–0.92	0.086	NR
	IM	L	0.11	0.04–0.30	0.001	DOWN
		L-A	0.11	0.04–0.32	0.001	DOWN
FASD2	SC	L	3.15	0.48–14.22	0.236	NR
		L-A	0.42	0.14–1.54	0.293	NR
	IM	L	0.31	0.18–0.52	<0.001	DOWN
		L-A	0.13	0.06–0.22	<0.001	DOWN
ELOVL5⁶	SC	L	3.50	1.69–8.08	0.101	NR
		L-A	0.30	0.16–0.59	0.203	NR

¹PPARG = peroxisome proliferator-activated receptor gamma; CEBPA = CAAT-enhancer binding protein alpha; SREBF1 = sterol regulatory element-binding factor 1; SCD = stearoyl-CoA desaturase; ACACA = acetyl-CoA carboxylase 1; LPL = lipoprotein lipase; FADS1 = fatty acid desaturase 1; FADS2 = fatty acid desaturase 2; ELOVL5 = fatty acid elongase 5.

²Treatments: L = 10% linseed; L-A = 5% linseed with 3.89% marine algae.

³FC = Fold change. The values reported represent the fold change relative to control group that has the value of 1.

⁴Std. Error. A range for Standard Error calculated by REST software.

⁵NR = not regulated.

⁶The relative gene expression of ELOVL5 in IM AT was not detected.