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. 2016 Jun 2;11(6):e0156599. doi: 10.1371/journal.pone.0156599

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© 2016 Penterling et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — (A) Increase of H4K16ac, H3K9ac, and H3K56ac in whole cell protein extracts of HeLa cells after siRNA transfection. Western blot images show levels of the histone modifications in cells transfected with scrambled siRNA (scr) and Jarid1A A1+A3 siRNA. Graphs indicate the means (+/- SD) from 5 independent experiments for H4K16ac and 3 independent experiments for H3K9ac and H3K56ac after quantitative analysis of Western blots. The effect of H4K16ac is statistically significant (p = 0.0035). (B) The amounts of the histones H3 and H4 are not affected by down-regulation of Jarid1A. Indicated are means (+/- SD) from 3 experiments.