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. 2016 Jun 2;11(6):e0156599. doi: 10.1371/journal.pone.0156599

Fig 6. Residual γH2AX foci and reporter plasmid-based DSB repair events are not affected by depletion of Jarid1A.

Fig 6

A) Mean background and residual foci number (+/- SEM) in at least 20 cells per sample after 5 Gy X-rays. Cells were fixed before and 24 h or 48 h after irradiation and indirect immunofluorescence was performed. Semi-automatic detection and characterization of spontaneous and residual γH2AX foci in the cells was performed by using the PlugIn FociPicker3D [28]. B) Relative number of GFP expressing cells, normalized to the frequency in untransfected HeLa pEJ (left panel) and pGC (right panel) control cells, after transfection of I-SceI expression plasmid into untreated cells and cells treated with scr siRNA or Jarid1A A1+A3 siRNA. Indicated are means ± SEM of 5 independent experiments.