Fig 2. Prestimulation with S. pneumoniae fails to modulate human platelet aggregation in response to subthreshold concentrations of TRAP.

Aggregation curves testing minimal TRAP concentration to induce aggregation are depicted in (A). Prior to stimulation with this subthreshold TRAP concentration, PRP was incubated with S. pneumoniae D39, ΔcpsD39, TIGR4 and 6303 for 10 minutes in a stirring cuvette (B). Aggregation curves are representative of 3 independent experiments using different donors.