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. 2016 Jun 2;11(6):e0156197. doi: 10.1371/journal.pone.0156197

Fig 4. Comparison of enzyme activities of wild type AlgL and mutant AlgL K194A, K194E, K245D, E312D, K319A, K194E/K245D, K245D/K319A, K194E/K245D/E312D, and K194E/K245D/E312D against two substrates (alginate from brown algae and acetylated alginate from Pseudomonas).

Fig 4

The purified enzymes were assayed for AlgL activity. AlgL activity was determined by the thiobarbituric acid method, as described in the “Experimental Procedures” section.