Fig 5. Simulations on the evolution of protein denaturation temperature under threshold selection for both thermodynamic stability and kinetic stability.
All simulations assume an environmental temperature of 37°C and use as starting state the final state of the K* = 10−2 and τ* = 0 simulation in the upper panel of Fig 3. τ* is kept at a zero value for 5000 simulation steps and then it is increased to impose different levels of selection for kinetic stability. Upper panel: simulations performed using different threshold values for the degradation half-life (τ*). Lower panel: final values of the denaturation temperature obtained in the simulations shown in the upper panel versus the threshold τ* used.