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. 2016 Jun 2;10(6):e0004747. doi: 10.1371/journal.pntd.0004747

Fig 5. TREM-2 deficiency reduces cellular responsiveness ex vivo.

Fig 5

Whole blood (A), bone marrow derived macrophages (BMDM; B) and alveolar macrophages (AM; C) of WT (black bars) and Trem-2-/- mice (white bars) were stimulated with medium, E.coli LPS (100 ng/ml) or heat-inactivated B. pseudomallei (107 CFU/ml or MOI of 50). Supernatant was collected after 20 h of stimulation and assayed for TNF-α. In addition, WT and Trem-2-/- BMDM (D) and AM (E) were incubated at 37°C with FITC labeled heat-inactivated B. pseudomallei after which time-dependent phagocytosis was determined. Data are presented as mean ± SEM and are representative of two or three independent experiments. n = 4 or 8 per group. * P< 0.05 (Mann-Whitney U test).