Fig. 1.

Derivation and maintenance of human adipose-derived mesenchymal stem cells from the stromal vascular fraction samples. The stromal vascular fractions (SVF) were made into single-cell suspensions and placed in MesenCult medium to maintain the adipose-derived mesenchymal stem cells in an undifferentiated state. At passage 0, the culture contained a heterogeneous population of cells with mixed morphology. However, at passages 1 and 2, the cultures became more homogeneous and exhibited a fibroblast-like morphology. Representative images of three different stromal vascular fraction samples from different passages are shown (original magnification, × 4).