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. 2015 Sep 29;136(4):414–425. doi: 10.1097/PRS.0000000000001635

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Copyright © 2015 by the American Society of Plastic Surgeons

This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially.

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Fig. 2. — The stromal vascular fraction contains adipose-derived mesenchymal stem cells. The stromal vascular fractions were made into single-cell suspensions and placed in colony-forming unit–fibroblast assays using the indicated starting cell numbers. After 14 days, colonies were fixed and stained with crystal violet. (Above, left) A representative photograph of a fibroblast colony derived from adipose-derived mesenchymal stem cells is shown (original magnification, × 4). (Above, right) The colony number is plotted against starting cell dose from different stromal vascular fraction samples (n = 7). As can be seen, culturing more cells will yield more colonies. (Below, left) The frequency of adipose-derived mesenchymal stem cells was calculated based on the number of colonies formed from each starting cell dose and plotted on a bar graph. As shown, at starting cell numbers over 1000 cells, the adipose-derived mesenchymal stem cell frequency is underestimated because of overcrowding of the plates, leading to less accurate colony counts. On average, only 2.62 ± 0.27 percent of each stromal vascular fraction sample contains adipose-derived mesenchymal stem cells. (Below, right) Colony-forming efficiency of adipose-derived mesenchymal stem cells in the stromal vascular fraction samples decreases at passage 3 (**p < 0.005) and passage 4 (***p < 0.0005) compared with the freshly isolated (***passage 0) samples. CFC-F, colony-forming cell–fibroblast.