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. 2015 Sep 29;136(4):414–425. doi: 10.1097/PRS.0000000000001635

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Copyright © 2015 by the American Society of Plastic Surgeons

This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially.

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Fig. 4. — Stromal vascular fraction samples contain multipotential adipose-derived mesenchymal stem cells. Multipotency of the adipose-derived mesenchymal stem cells present in the stromal vascular fractions was examined. Stromal vascular fraction cells were placed in adipogenic, osteogenic, and chondrogenic differentiation culture conditions. Cells were fixed and stained with Oil Red O to identify presence of adipocytes (left) or Alizarin Red S to distinguish differentiation to osteoblasts and bone matrix mineralization and calcium deposition (center). Cells grown in chondrogenic medium (right) show positive staining for Alcian Blue 8GX, indicative of chondrogenic differentiation.