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Nested and Cytb PCRs for Plasmodium detection in An. triannulatus. One % agarose gel. Lanes: MW: molecular weight, PC: Positive control—Plasmodium vivax DNA, NC: negative control (no DNA). (a). Plasmodium genus-specific Nested PCR. 1–11: DNA from An. triannulatus from Puerto Libertador (PLT), (b). PCR-Cytb, 1–13: An. triannulatus from various localities. Lane 5: positive specimen from PLT, equivalent to sample in lane 2 of gel (a).
