Table 3.
Natural infection on Anopheles triannulatus from Northwestern and Southeastern Colombia.
| Region/locality/collection site |
No. of An. triannulatus analyzed |
Plasmodium speciesa: infection ratesb (95% CI) |
ELISA test | PCR | ||
|---|---|---|---|---|---|---|
| First | Second | Nested | Cytb | |||
| (confirmation) | ||||||
| NW | ||||||
| El Bagre-BAG, La Capilla | 82 |
PvVK247: 1.51 (0.04–8.15) |
Positive | Negative | Positive | Negative |
| Puerto Libertador-PLT, Juan José | 83 | Pf: 1.20 (0.03–6.53) | Negative | Negative | Positive | Positive |
| SE | ||||||
| Puerto Nariño (PNA), Puerto Rico | 83 | Pf: 1.20 (0.03–6.53) | Positive | Negative | Positive∗ | Negative |
| Tarapaca (TAR), Ventura | 118 | Pf: 0.85 (0.02–4.63) | Positive | Negative | Positive∗ | Negative |
The table shows only results for those localities where naturally infected An. triannulatus were detected. a Plasmodium species: PvVK247-P. vivax VK247, PvVK210-P. vivax VK210, Pf: P. falciparum.bNumber of positive An. triannulatus (np) per number of total analyzed (nt) per 100, determined for each locality, (IR = (np/nt) × 100). ∗Positive result only in the nested PCR that detects Plasmodium genus, negative in the nested PCR that detects Plasmodium species. Due to the limitations on the available tests for detecting naturally infected anopheline mosquitoes (see discussion), positive specimens were determined as those given a positive result in at least two of the tests.