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. 2015 Dec 28;7(8):8676–8687. doi: 10.18632/oncotarget.6779

Figure 2. Resistant BCPAP cells do not exhibit oscillatory inhibition of phospho-ERK and have increased CRAF dimers.

Figure 2

(A–D) Nthy-ori 3–1, BCPAP, 8505c, and resistant BCPAP cells were subjected to 10 μM VMR for various time points and cytoplasmic extracts were collected and analyzed by Western blot for expression of phospho-ERK1/2, CRAF, phospho-CRAF, and GAPDH (loading control). (E) Cytoplasmic extracts from thyroid cell lines were collected after 24 hours untreated and treated with 10 μM VMR and analyzed by Western blot for comparison of CRAF expression. After being subjected to the same 24 hour treatment, thyroid cell lines were collected and lysed using 1% NP-40 buffer. Lysates were subjected to immunoprecipitation using anti-CRAF antibody, SDS-PAGE, and Western blot analysis for CRAF.