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. 2015 Dec 24;7(8):8756–8770. doi: 10.18632/oncotarget.6752

Table 1. Primer sequences for MAP3K11 luciferase reporters.

Name Sequence Region Cutting Site
pmirGLO-F1-Fwd GCTAGCACATCACGCTCTGCTCCTG 2726–3109 Nhe I
pmirGLO-F1-Rev GTCGACCCAGCTCCTGTTCCAGTGTA 2726–3109 Sal I
pmirGLO-F2-Fwd GCTAGCTTGGGGGTCAGGAACACTAC 3157–3572 Nhe I
pmirGLO-F2-Rev GTCGACGTGGGGAGACAGCTTTTGAG 3157–3572 Sal I
pmirGLO-FL-Fwd GCTAGCAGGGCACAGACCAAAGACAT 2976–3572 Nhe I
pmirGLO-FL-Rev GTCGACGTGGGGAGACAGCTTTTGAG 2976–3572 Sal I
pmirGLO-Mut-F1-Fwd GGCTGACCCAGCTCCTGTTAACGTGTATGCTGTGACTCCTC 2726–3109 N/A
pmirGLO-Mut-F1-Rev GAGGAGTCACAGCATACACGTTAACAGGAGCTGGGTCAGCC 2726–3109 N/A
pmirGLO-Mut-F2-Fwd CAGGAAGCCTTCACACGTTAAGGGGGACCTGCGCC 3157–3572 N/A
pmirGLO-Mut-F2-Rev GGCGCAGGTCCCCCTTAACGTGTGAAGGCTTCCTG 3157–3572 N/A