Table 1. Primer sequences for MAP3K11 luciferase reporters.
Name | Sequence | Region | Cutting Site |
---|---|---|---|
pmirGLO-F1-Fwd | GCTAGCACATCACGCTCTGCTCCTG | 2726–3109 | Nhe I |
pmirGLO-F1-Rev | GTCGACCCAGCTCCTGTTCCAGTGTA | 2726–3109 | Sal I |
pmirGLO-F2-Fwd | GCTAGCTTGGGGGTCAGGAACACTAC | 3157–3572 | Nhe I |
pmirGLO-F2-Rev | GTCGACGTGGGGAGACAGCTTTTGAG | 3157–3572 | Sal I |
pmirGLO-FL-Fwd | GCTAGCAGGGCACAGACCAAAGACAT | 2976–3572 | Nhe I |
pmirGLO-FL-Rev | GTCGACGTGGGGAGACAGCTTTTGAG | 2976–3572 | Sal I |
pmirGLO-Mut-F1-Fwd | GGCTGACCCAGCTCCTGTTAACGTGTATGCTGTGACTCCTC | 2726–3109 | N/A |
pmirGLO-Mut-F1-Rev | GAGGAGTCACAGCATACACGTTAACAGGAGCTGGGTCAGCC | 2726–3109 | N/A |
pmirGLO-Mut-F2-Fwd | CAGGAAGCCTTCACACGTTAAGGGGGACCTGCGCC | 3157–3572 | N/A |
pmirGLO-Mut-F2-Rev | GGCGCAGGTCCCCCTTAACGTGTGAAGGCTTCCTG | 3157–3572 | N/A |