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. 2016 Feb 1;7(8):8797–8808. doi: 10.18632/oncotarget.7122

Figure 4. Inhibition of EV71 infection by Knockdown of ERp57 at early stage.

Figure 4

A. Specific siRNAs targeting ERp57 were transfected into RD cells, and the effect of knockdown was determined by qRT-PCR 48 hours post-transfection. B. The ERp57 protein level detected by si-ERp57-1 knockdown. C. Knockdown of ERp57 did not affect EV71 entry. Forty eight hours post-transfection with siRNAs, cells were infected with EV71 at MOI of 1 for 1 hour (time point was set as 0 hour), and cells were washed with PBS for three times. The intracellular viral RNA level was determined by qRT-PCR. The mean value of VP1/GAPDH ratio was set as 1 in control. D. and E. The intracellular (D) and extracellular (E) viral RNA levels upon ERp57 knockdown at different time points. The total RNA was isolated from infected cells and corresponding media at different time points, and quantified by quantitative RT-PCR. At time point 0 hour, the intracellular viral RNA level (virions just entered the cells) were quantitated by qRT-PCR *, p < 0.05; **, p < 0.01; ***, p < 0.001.