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. 2016 Feb 1;7(8):8797–8808. doi: 10.18632/oncotarget.7122

Figure 5. Inhibition of EV71 IRES activity by OM or knockdown of ERp57.

Figure 5

A. Schematic diagram of plasmids pIRES and pRF for measuring IRES activity. B. OM inhibited IRES activity in a dose dependent manner. Cells were transfected with pIRES or pRF for 24 hours, and then treated without or with OM for 12 hour followed by luciferase assays. C. Knockdown of ERp57 reduced IRES activity. Cells were transfected with scramble siRNA or si-ERp57-1. Twenty four hours post-transfection, the cells were then transfected with pIRES or pRF for another 24 hours and applied for luciferase assays. The ratio of FLuc to RLuc activity was set as 1 when cells were transfected with pRL reporter plasmid in the control group (without OM treatment or transfected with scrambled siRNA). *, p < 0.05; ***, p < 0.001.