Table 2. Differentially expressed proteins identified by 2-DE/MS analysis in RD cells treated without/with OM (At a 2-fold difference cutoff in intensity, xx spots were selected for further MALD-TOF-MS/MS analysis, 18 proteins were finally identified).
| Spots No.a | Protein name | Abbreviation | NCBI accession no. | Theoretical molecular mass(Da) | PI | Protein core (CI) | Masses matched/ searched | Sequencecoverage (%) | ‘-Fold changeb |
|---|---|---|---|---|---|---|---|---|---|
| Structural molecule activity | |||||||||
| 1 | Rho-associated protein kinase 2 | ROCK2 | O75116 | 161939 | 5.75 | 69 | 9/13 | 5 | 11.7↓ |
| 24 | Prelamin-A/C | LMNA | P02545 | 74380 | 6.57 | 97 | 15/36 | 22 | 4.1↓ |
| 26 | Annexin A7 | ANXA7 | P20073 | 52991 | 5.52 | 71 | 8/21 | 15 | 3.7↑ |
| Metabolism | |||||||||
| 4 | Reticulocalbin-3 | RCN3 | Q96D15 | 37470 | 4.74 | 94 | 8/27 | 32 | 18.2↓ |
| 5 | Reticulocalbin-1 | RCN1 | Q15293 | 38866 | 4.86 | 115 | 12/34 | 34 | 15.3↓ |
| 6 | Calumenin | CALU | O43852 | 37198 | 4.47 | 80 | 9/30 | 26 | 4.3↓ |
| 7 | Proteasome subunit alpha type-3 | PSMA3 | P25788 | 28643 | 5.19 | 60 | 6/26 | 20 | 3.8↑ |
| 15 | N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 | DDAH2 | O95865 | 29911 | 5.66 | 59 | 6/28 | 18 | 3.6↑ |
| 21 | Mitochondrial inner membrane protein | IMMT | Q16891 | 84025 | 6.08 | 117 | 14/28 | 16 | 3.3↓ |
| 37 | 26S protease regulatory subunit 10B | PSMC6 | P62333 | 44430 | 7.1 | 58 | 6/14 | 13 | 3.3↑ |
| Cytoskeleton and transport proteins | |||||||||
| 8 | Actin, cytoplasmic 1 | ACTB | P60709 | 42052 | 5.29 | 111 | 9/29 | 26 | 3.8↑ |
| 22 | Vinculin | VCL | P18206 | 124292 | 5.5 | 96 | 14/27 | 11 | 3.0↑ |
| Gene transcription | |||||||||
| 39 | Far upstream element-binding protein 1 | FUBP1 | Q96AE4 | 67690 | 7.18 | 110 | 11/21 | 17 | 3.1↑ |
| Heat shock proteins and chaperones | |||||||||
| 17 | Protein disulfide-isomerase A3 | ERp57 | P30101 | 57146 | 5.98 | 76 | 11/31 | 17 | 13↓ |
| 18 | T-complex protein 1 subunit alpha | TCP1 | P17987 | 60819 | 5.8 | 77 | 9/24 | 16 | 3.4↓ |
| 27 | T-complex protein 1 subunit beta | CCT2 | P78371 | 57794 | 6.01 | 121 | 13/29 | 22 | 3.9↓ |
| Cell proliferation, metastasis, and signal transduction | |||||||||
| 33 | Septin-11 | SEPT11 | Q9NVA2 | 49652 | 6.36 | 114 | 12/22 | 22 | 8.5↑ |
| 34 | Fascin | FSCN1 | Q16658 | 55123 | 6.84 | 103 | 10/24 | 18 | 10.8↓ |
a
Spot numbers are shown in Figure 2.
b
The spot intensities were quantified using PDQuest software (Bio-Rad). The average -fold change of spot intensity for each protein was calculated from three independent experiments (DMSO-treated RD versus OM-treated RD). ↑, increase; ↓, decrease.