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. 2016 Jan 7;7(8):8896–8907. doi: 10.18632/oncotarget.6840

Figure 2. Bufalin suppressed cervical cancer cell migration and invasion by regulating EMT-associated proteins.

Figure 2

A. Detection of migration by scratching assay. B, Quantitative analysis of migration speed using migration index (P < 0.05). Error bars = 95% CIs. C, Detection of cell migration and invasion by using a high throughput screening multi-well insert 24-well two-chamber plates. D-E, Quantitative analysis of invaded cells (P < 0.05). Error bars = 95% CIs. F, Immunoblotting analysis of EMT-associated proteins. G-H, Representative images showing Bufalin inhibited the expression of Snail1 in cell nucleus with immunofluorescence staining (×1000). Blue dye DAPI indicates nucleus.