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. 2016 Jan 9;7(8):8916–8930. doi: 10.18632/oncotarget.6866

Figure 1. Cr(VI) suppresses the protein level of Gene 33.

Figure 1

A. BEAS-2B cells were treated with the indicated concentrations of Cr(VI) for 72 hours and harvested for total cellular proteins with 1x sample buffer. Total cellular proteins were subjected to Western blot as described in Materials and Methods with antibodies toward the indicated proteins. GAPDH or β-actin was used a loading control. B. Same as A with 48h instead. C. BEAS-2B cells were treated with 5 μM Cr(VI) for indicated time periods and then processed as in A. D. BEAS-2B cells were transfected with either a scrambled siRNA (control) or an siRNA targeting Gene 33 as described in Materials and Methods. Forty eight hour after transfection, cells were treated with 20% FBS (F) or Cr(VI) for additional 48 hours and processed as in A. E. A549 cells were left untreated or treated with 20% FBS or 5 μM Cr(VI) for 48 hours. Cells were then processed as in A. F. BEAS-2B cells were cultured for 2 months (passed every three days) using media containing 0, 0.25, or 0.5 μM Cr(VI). Cells were harvested and processed at in A.