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. 2016 Jan 9;7(8):8916–8930. doi: 10.18632/oncotarget.6866

Figure 4. Gene 33 depletion elevates Cr(VI)-induced DNA damage but does not affect cell cycle progression.

Figure 4

A.-C. BEAS-2B A. & B. or A549 C. cells were transfected with scrambled (Ctrl) siRNA oligo or an siRNA oligo for Gene 33 (G33). Forty eight hours after transfection, cells were either left untreated or treated with 5 μM Cr(VI) for 6 hours (A&B) or 24 hours C. & D.. Cells were harvested for total proteins at the end of the treatments. Total protein lysates were subjected to Western blot with antibodies against the indicated proteins. D. & E. BEAS-2B cells transfected with either control or Gene 33 siRNA oligos as in A, B&C. Cells were then passed 48 hours after transfection. Twenty four hours later, Cells were stained with DAPI followed by cell cycle analysis. Data are presented as mean +/− SD. An aliquot of cells from each transfection was collected for Western blot to check the protein level of Gene 33 (F).