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. 2016 Jan 17;7(8):9007–9016. doi: 10.18632/oncotarget.6928

Figure 2. Cryab promoted the invasion and metastasis of OS cells in vitro and vivo.

Figure 2

A. RT-PCR and immunoblotting analyzed the expression of Cryab in hFOB1.19, MG-63, U-2OS, Saos and HOS cell lines (p < 0.05); (B and C) The expression of Cryab were regulated in MG-63 and U-2OS cells by RNA interference; D. The wound-healing assay revealed that an evident delay in the wound closure rate of MG-63/U-2OS-vshRNA-Cryab cells was found at 24 and 48h, compared with MG-63/U-2OS-Mock cells (bar = 100 μm); E. The down-regulation of Cryab has no influence on cell proliferation (p > 0.05); F. Matrigel invasion assays showed that down-regulation of Cryab was accompanied by a descend invasion of OS cells (bar = 100μm); G. Serial sections from mouse lung showed the metastasis ability of cancer cells expressing different Cryab (bar = 50μm); H. The tumor volume of cancer cells expressing different level of Cryab.