Figure 3. High level of Cryab induces OS progression via up-regulation of MMP9 expression through ERK1/2 signaling.

A. The secretion and expression of MMP9 were inhibited by the Cryab specific RNA interference in OS cells; B. The phosphorylation level of MEK and ERK1/2 were reduced significantly in MG-63/U-2OS-vshRNA-Cryab cells, compared with MG-63/U-2OS-Mock cells; C. The level of ERK1/2 activity was elevated, and the expression of MMP9 were up-regulated by Cryab cDNA transfection in HOS cells; D. Immunofluorescent staining showed that the expression of Cryab was in line with the activation of ERK1/2; E. The inhibition of ERK1/2 phosphorylation by U0126 was associated with the inhibition of secretion of MMP9 and OS cells invasion (bar = 200μm).