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. 2016 Jan 27;7(8):9026–9040. doi: 10.18632/oncotarget.7030

Figure 2. PN reduces percentages of MITF-positive cells and diminishes levels of MITF-M protein and phosphorylated p65/NF-κB.

Figure 2

A. Percentages of MITF (total)-positive and Melan-A-positive cells after treatment with 20 μM PN for 4 hours were assessed by flow cytometry. The numbers are representative percentages of MITF- or Melan-A-positive cells. B. Melanoma cells were treated with 10 μM PN for 24 hours or with 20 μM PN for 4 hours, and changes in the levels of MITF-M and phosphorylated p65 (p-p65) in whole-cell lysates were determined by Western blotting. C. MITF-M levels in cytosolic and nuclear fractions were analyzed by Western blotting after 3 hours of incubation with 20 μM PN. Equal loading was confirmed by β-actin (whole-cell lysates and cytosolic fraction) and lamin B (nuclear fraction). Representative results are shown.