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. 2016 Jan 25;7(8):9271–9287. doi: 10.18632/oncotarget.7005

Figure 3. MiR-497 overexpression reduces proliferation of chemoresistant NB cells and induces apoptosis in MYCN-amplified NB cells.

Figure 3

(A) Proliferation time course comparing miR-497 versus miR-Control (25 nM) reverse transfected in CHLA-90 and SK-N-AS cells (both non-MYCN amplified) or SK-N-BE(2) and LA1-5s cells (both MYCN amplified). (B) Representative images of nuclear morphology assessment at 96 h post-transfection with Hoechst staining in miR-Control and miR-497 (25 nM) reverse transfected NB cell lines. Arrowheads point at condensed or fragmented nuclei. (C) Quantification of apoptosis was performed from 4 representative images of 3 replicates per condition. (D) Caspase-3/7 activity assays and (E) representative Western blot of PARP protein at 72 h post-transfection. SK-N-BE(2) and LA1-5s cells were reverse transfected with 25 nM of miR-Control or miR-497. Data represent mean ± SEM of three independent experiments *, ** or *** indicated significant differences comparing miR-497 versus miR-Control at p < 0.05, p < 0.01 or p < 0.001, respectively.