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. 2016 Jan 25;7(8):9271–9287. doi: 10.18632/oncotarget.7005

Figure 5. Overexpression of miR-497 in vivo reduces permeability of blood vessels.

Figure 5

(A) Representative images of mice bearing NB xenografts with and without doxycycline treatment and quantification of mice with hemorrhagic tumors. (B) Representative images of dissected tumors (bar indicates 1 cm). (C) Hemorrhagic area was determined developing endogenous tissue peroxidase (mostly from red blood cells) using DAB precipitate deposition. Representative histology images of dissected tumors and quantification of hemorrhage areas of the images. (D) Vascularization was determined using an anti-CD31 (PECAM1) antibody and Envision anti-Rabbit HRP-conjugated secondary antibody complex. Representative histology images of dissected tumors and quantification of vascular areas of the images. Quantification of both vascularization and hemorrhaging was made from 3 representative images of 4 independent tumors per group.