Figure 7. Physalin A inhibits phosphorylation of JAKs in human NSCLC cells in a dose- and time-dependent manner.

(A) H292 cells were incubated with the indicated concentrations of physalin A for 4 h, and the phosphorylation levels of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) and Src were determined by Western blotting. (B) H1975 and H358 cells were incubated with 15 μM physalin A for 4 h, and the phosphorylation levels of Jak2 and Jak3 were determined by Western blotting. (C) H292 cells were incubated with 15 μM physalin A for 0.5, 1, 2 and 4 h, and Western blotting was performed to determine the time-dependent phosphorylation level of Jak1, Jak2, Jak3, Tyk2 and SRC and STAT3. β-actin was used as a loading control.