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. 2016 Jan 29;7(8):9513–9524. doi: 10.18632/oncotarget.7069

Figure 5. Suppression of YAP does not decrease TRAIL-induced apoptosis in cells that are stably silenced for CABYR-a/b.

Figure 5

Knockdown of YAP using siRNA for 48 h in H460 and A549 cells that were stable silenced CABYR-a/b. Scramble siRNA was used as a control. These cells were treated with 20 ng/mL TRAIL for 6 h in H460 cells or 200 ng/mL for 24 h in A549 cells. The percentage of apoptosis was measured using PE/7-AAD staining and then was analyzed by flow cytometry (A). Histograms demonstrate the apoptotic rates of the cells (B). The results represent the means of at least three independent experiments. The expression of YAP, p73 and DR5 was detected by western blotting (C). β-Actin was used as an internal control to ensure equal protein loading.