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. 2015 Dec 19;7(8):9525–9537. doi: 10.18632/oncotarget.6672

Figure 4. The inhibition of Notch pathway attenuated the cancer stem cell properties of CD90+ cells.

Figure 4

A. In CD90+ cells transinfected with Lv-Notch1-si the expression levels of NICD, Hes1 and Hey1 were decreased by qRT-PCR and WB (**p < 0.01, ***P < 0.005, t test). B. The efficiency of tumor formation of CD90+ PLC/PRF/5 cells after transinfection with Lv-Notch1-si (4/12) or control lentivector (0/12, as shown in Supplementary Table 2). Red arrows in the left graph indicated the sites of cells injection, while in the right graph indicated the site of tumor formation. C. By colony formation CD90+ cells transinfected with Lv-Notch1-si had a significantly lower proliferation rate when compared with CD90− cells (**p < 0.01, ***P < 0.005, t test). D, E. The invasive (D) and migratory (E) activities of CD90+ cells from PLC/PRF/5 and Huh7 could be reduced after downregulation of NICD (**p < 0.01, ***P < 0.005, t test) F. Western blotting analysis of stem cell marker Sox2 and Nanog in CD90+ PLC/PRF/5 cells when attenuated the expression of NICD.