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. 2016 Feb 1;7(9):9680–9691. doi: 10.18632/oncotarget.7109

Figure 1. EGFRvIII promotes GBP1 expression.

Figure 1

The expression of GBP1 was analyzed by semiquantitative RT-PCR A. and Western blot B. in U87-parental (p), U87-DK and U87-EGFRvIII cells which were serum starved for 48 hr. C. After 24 h of serum starvation, U87-P, U87-DK and U87-EGFRvIII cells were treated with AD or CHX for an additional 24 h. GBP1 and GAPDH mRNA was measured by RT-PCR. D. U87-P, U87-DK and U87-EGFRvIII cells were transfected with pGL3-237 and pRL-TK for 24 h and then serum starved for 24 h. Firefly and Renilla luciferase activities were measured, and promoter activity is presented as the fold induction of RLU (values of firefly luciferase unit/values of Renilla) as compared with control. The results are expressed as the mean of three independent experiments ± SD. *, P < 0.01.