Figure 6. GBP1 overexpression increases glioma tumor growth rate in mice.
A. WST-1 assay was performed to examine the effect of GBP1 overexpression on U87 cell proliferation. Expression level of GBP1 was analyzed by Western blot. B. U87-lacZ and -GBP1 flank xenograft tumor volume (mm3) was measured at the indicated time in nude mice. 5 × 106 cells/mouse, 6 mice/group. *, P < 0.05. C. H&E staining of brain sections on day 20 after intracranial inoculation of U87-lacZ (left) or U87-GBP1 (right) (1 × 106 cells/mouse, 6 mice/group). Shown are representative brain slices from tumor-bearing mice. Tumor margins are delineated using a dotted line. Bars, 50 μm. D. Kaplan-Meier survival curve of mice after intracranial implantation of U87-LacZ or U87-GBP1 (1 × 105 cells/mouse, 8 mice/group). Statistical comparisons were performed using a log-rank test. p = 0.0165.