Figure 5. GATA1 modulates VEGF expression and VEGF-induced HUVEC proliferation, migration and tube formation as well as angiogenesis through SET7.

(A) Luciferase reporter assays in MCF7 cells cotransfected with the VEGF-Luc reporter, GATA1 and SET7 shRNA1, SET7 shRNA2 or control shRNA. Representative Western blot shows the expression of GATA1 and SET7. (B) Real-time RT-PCR analysis of the VEGF₁₂₁ and VEGF₁₆₅ expression in MCF7 cells stably infected with lentiviruses carrying GATA1 and SET7 shRNA. Representative Western blot indicates the expression of GATA1 and SET7. (C) Cell proliferation assays in HUVEC cells cultured in conditioned medium from MCF7 cells stably infected as in (B). (D) Wound healing assays for HUVEC cells cultured in conditioned medium from MCF7 cells stably infected as in (B). Scale bar: 100 μm. (E) Tube formation assays for HUVEC cells cultured in conditioned medium from MCF7 cells stably infected as in (B). Scale bar: 100 μm. (F) CAM assays with conditioned medium from MCF7 cells stably infected as in (B). All values shown are mean ± SD of triplicate measurements and have been repeated 3 times with similar results (A–F). *P < 0.05, **P < 0.01 versus corresponding control.