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. 2016 Feb 2;7(9):9859–9875. doi: 10.18632/oncotarget.7126

Figure 7. GATA1 and SET7 positively correlate with VEGF expression and are independent poor prognostic markers for breast cancer.

Figure 7

(A) Representative immunohistochemical staining of SET7 in human cancerous breast tissues and adjacent normal breast tissues. Scale bar: 25 μm. SET7 expression scores were plotted and compared (Mann-Whitney U test). (B) Representative immunohistochemical staining of GATA1, SET7, VEGF and CD31 in human breast cancer samples. Scale bar: 25 μm. For the quantification of microvessel density, pictures from eight areas in each tissue were taken and the vessel number was counted. The correlation of GATA1 or SET7 with VEGF or microvessel number (positive CD31 staining) is shown. The P value was generated using Pearson's χ2 test (GATA1, SET7 and VEGF) and Wilcoxon ranked sum test (CD31). (C) Kaplan-Meier estimates of disease-free survival (left panel) and overall survival (right panel) of breast cancer patients. Marks on graph lines represent censored samples. (D) Proposed model for GATA1 modulation of VEGF expression as well as its tumor-promoting function. GATA1 recruits SET7, TFIIB and RNA Pol II to VEGF core promoter, resulting in increased VEGF transcription and secretion in breast cancer cells. The cancer cell-secreted VEGF enhances vascular endothelial cell proliferation, migration and tube formation as well as tumor angiogenesis and growth.