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. 2016 Jan 28;7(9):10037–10050. doi: 10.18632/oncotarget.7048

Figure 5. CUL4A is a direct target of miR-9 and miR-137.

Figure 5

A. Four miRNAs were computationally predicted using two independent miRNA databases. B. Western blotting results showed that the overexpression of miR-9 and miR-137 significantly reduced CUL4A protein levels in HGC-27, SGC-7901 and BGC-823 cells; whereas, miR-103 and miR-107 had no effect on CUL4A protein levels. C. Quantitative PCR results of CUL4A mRNA levels after transfecting GC cells with miR-9 and miR-137 mimics. D. Quantitative PCR products containing predicted wild-type or mutant CUL4A 3′-UTR miRNA binding sites were inserted into luciferase reporter plasmids. E. In luciferase activity assays, miR-9 and miR-137 suppressed luciferase activity of the wild-type but not mutant CUL4A 3′-UTR constructs in 293 cells. Each bar represents the mean±SD of three independent experiments. *p-value <0.05.