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. 2016 Feb 3;7(9):10104–10116. doi: 10.18632/oncotarget.7173

Figure 3. Effect of the GAS5 HREM DNA oligonucleotide on UV-C-induced cell death of breast cancer cells.

Figure 3

Cells (n = 4 cultures) were transfected with the GAS5 HREM DNA oligonucleotide (HREM) or scrambled (scram) or stem loop (SL) control DNA oligonucleotides; transfections were also conducted with the plasmids pcDNA3.GAS5 (encodes mature GAS5 lncRNA) and pcDNA3 (empty control vector) as positive controls. Cells were harvested at 24 h post-transfection, irradiated with UV-C light and re-plated for assessment of cell survival after a further 48 h. The GAS5 HREM oligonucleotide increased UV-C induced apoptosis and the associated loss of cell viability in MCF7 (panels A & B, respectively), T-47D (panels E & F, respectively) and MDA-MB-231 (panels I & J, respectively) cells, similar to the action of GAS5 lncRNA (panels C & D, respectively for MCF7 cells; G & H, respectively for T-47D cells; and K & L, respectively, for MDA-MB-231 cells). *P < 0.05, **P < 0.01 and ***P < 0.001 versus scram and SL for HREM or versus pcDNA3 for pcDNA3.GAS5 (one-way ANOVA and Bonferroni's MCT).