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. 2016 Feb 4;7(9):10203–10214. doi: 10.18632/oncotarget.7190

Figure 1. Bisleuconothine A is a potent and selective inhibitor of the canonical Wnt pathway.

Figure 1

A. The inhibitory activity of vinorelbine, Bisleuconothine A (BLA) and its derivatives on Wnt signaling. HEK293W cells in 96-well plates were incubated with indicated concentrations of different compounds for 24 h. The luciferase activity was then measured and normalized to the activity of the Renilla. The values represent the mean ± S.D. (n=3). B. HEK293T cells in 96-well plates were transiently transfected with Wnt1 (64 ng), SuperTop Flash construct (80 ng) and Renilla (8 ng) in each well. After incubation for 3 h, cells were treated with indicated concentrations of Bisleuconothine A for 24 h, and the luciferase activity was then measured and normalized to the activity of the Renilla. The values represent the mean ± S.D. (n=3). C. HEK293T cells in 96-well plates were transiently transfected with NF-κB Luc (150 ng) and Renilla (15 ng) in each well. After incubation for 3 h, cells were pretreated with indicated concentrations of Bisleuconothine A and stimulated with 25 ng/ml TNF-α for 24 h, and the luciferase activity was then measured and normalized to the activity of the Renilla. The values represent the mean ± S.D. (n=3). The significance was determined by Student's t test (*p<0.05, **p<0.01 vs. control).