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. 2016 Feb 3;7(9):10228–10242. doi: 10.18632/oncotarget.7169

Figure 2. The endogenous and manipulated transcriptional profiles of NMU and NMURs in the various endometrial cancer cell lines.

Figure 2

The endogenous transcripts of (A) NMU, (B) NMUR1 and NMUR2 were determined in Ishikawa (from the grade I cancer), RL95-2 (from the grade II cancer) and HEC1A (from the grade II cancer). NMU knockdown efficiencies were evaluated by determining the changes in (C) the transcript and (D) the protein levels of NMU in Ishikawa and RL95-2. NMUR2 overexpression efficiency in HEC1A was evaluated by determining the changes in (E) the transcript and (F) the protein levels of NMUR2. The transcript of each gene was quantified by real-time PCR and then normalized against ACTB. Data are shown as the mean ± SD. *p < 0.05.