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. 2016 Feb 3;7(9):10228–10242. doi: 10.18632/oncotarget.7169

Figure 3. NMU signaling positively regulates cell motility in the grade II endometrial cancer cell lines.

Figure 3

Transwell migration assays were performed to evaluate NMU signaling-mediated migration ability in (A) Ishikawa, (B) RL95-2 and (C) HEC1A cells. Transwell chambers coated with Matrigel were used to assay NMU signaling-mediated invasiveness in (D) Ishikawa, (E) RL95-2 and (F) HEC1A cells. 100 nM NMU was used for the exogenouse NMU treatment of HEC1A cells. For all assays, a represented image was presented in the upper panel. To obtain the average cell number (lower panel), at least five fields of transmigrated cells were randomly photographed and counted for each individual experiment. The results are shown as the mean ± SD. *p < 0.05.