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. 2016 Feb 3;7(9):10228–10242. doi: 10.18632/oncotarget.7169

Figure 4. Effects of NMU knockdown on the cell growth and morphology of endometrial cancer cells.

Figure 4

(A) Ishikawa and (B) RL95-2 cells with or without NMU knockdown were cultured in the growth medium. (C) HEC1A cells with eGFP- or NMUR2-overexpression were cultured in the growth medium supplemented without or with 100 nM NMU. The growth rates were compared using the AlamarBlue assay. The fluorescence value of the cells on day 0 (D0) served as the one-fold control. Data are shown as the mean ± SD. *p < 0.05. The morphologies of (D) Ishikawa, RL95-2 and (E) HEC1A cells were observed on Day 5 of culture. Scale bar, 100 μm.