Figure 10. CAGE confers resistance to EGFR inhibitors.

(A) Shows CAGE deletion constructs. (B) Each construct (1 μg) was transiently transfected into Malme3M cells. At 48 h after transfection, cell lysates were immunoprecipitated with the indicated antibody (2 μg/ml), followed by immunoblot analysis. Cell lysates were also subjected to immunoblot analysis, (C) Malme3M^R cells were transiently transfected with the indicated siRNA (each at 10 nM). At 48 h after transfection, cell lysates were subjected to immunoblot analysis. (D) Each construct (1 μg) was transiently transfected into Malme3M cells. At 24 h after transfection, cells were then treated with the indicated anti-cancer drugs for 24 h, followed by MTT assays.