. 2016 Feb 4;7(9):10322–10331. doi: 10.18632/oncotarget.7188

Table 1. Real-time qPCR and methylation-specific PCR primers.

A. Real-Time qPCR primers
Gene	NCBI ref seq	Sequence 5′→3′	Ta.°C	Amplicon. bp
Dnmt1	NM_001130823	fw TACCTGGACGACCCTGACCTC	60	103
		rv CGTTGGCATCAAAGATGGACA
Dnmt3a	NM_175629	fw GACAAGAATGCCACCAAAGC	60	190
		rv CGTCTCCGAACCACATGAC
Dnmt3b	NM_006892	fw GGCAAGTTCTCCGAGGTCTCTG	60	113
		rv TGGTACATGGCTTTTCGATAGGA

B. Methylation specific PCR primers
Gene	sequence 5′→3′	Ta.°C	Amplicon. bp
CDH13 meth	Fw TCGCGGGGTTCGTTTTTCGC	66	243
CDH13 meth	Rv GACGTTTTCATTCATACACGCG
CDH13 unmeth	Fw TTGTGGGGTTGTTTTTTGT	55	242
CDH13 unmeth	Rv AACTTTTCATTCATACACACA
APC meth	Fw TATTGCGGAGTGCGGGTC	64	98
APC meth	Rv TCGACGAACTCCCGACGA
APC unmeth	Fw GTGTTTTATTGTGGAGTGTGGGTT	62	108
APC unmeth	Rv CCAATCAACAAACTCCCAACAA
RUNX3 meth	Fw TTACGAGGGGCGGTCGTACGCGGG	71	220
RUNX3 meth	Rv AAAACGACCGACGCGAACGCCTCC
RUNX3 unmeth	Fw TTATGAGGGGTGGTTGTATGTGGG	64	220
RUNX3 unmeth	Rv AAAACAACCAACACAAACACCTCC
MGMT meth	Fw TTTCGACGTTCGTAGGTTTTCGC	64	81
MGMT meth	Rv GCACTCTTCCGAAAACGAAACG
MGMT unmeth	Fw TTTGTGTTTTGATGTTTGTAGGTTTTTGT	64	93
MGMT unmeth	Rv AACTCCACACTCTTCCAAAAACAAAACA
MLH1 meth	Fw ACGTAGACGTTTTATTAGGGTCGC	56	115
MLH1 meth	Rv CCTCATCGTAACTACCCGCG
MLH1 unmeth	Fw TTTTGATGTAGATGTTTTATTAGGGTTGT	56	124
MLH1 unmeth	Rv ACCACCTCATCATAACTACCCACA