Skip to main content
. 2016 Feb 8;7(9):10332–10344. doi: 10.18632/oncotarget.7243

Figure 5. Blocking of NKG2D binding with its ligands results in impaired cytotoxic activity of CIK cells against tumor cell lines (MGC803, HGC27, RKO, and SW480).

Figure 5

A. All the tumor cell lines were observed for MIC A/B expression. Besides, the lytic activity of CIK cells, with or without blocking its recognition receptor, NKG2D, was assessed by cytotoxicity assay. B. Meanwhile, all the above cell lines were detected for the levels of ULBP-2, another crucial ligand for NKG2D. Antibody-blockade of ULBP-2 was conducted to observe cytotoxicity of CIK cells against all the above cells that differentially express ULBP-2. filled isotype staining; bold line MIC A/B− or ULBP-2-stained tumor cell. All the data on the curves represent three independent experiments using CIK cells from the same donor and are shown as Mean±SEM.