Figure 4.

(A) Western blot detection of UMP/CMPK1 and 2 in the parental and CDV^R SiHa, HeLa and HaCaT cells. (B) Fold-change (parental/CDV) of UMP/CMPK1 and 2 gene expression based on relative quantification compare to actin gene expression. The bars indicate the statistical difference between SiHa, HeLa and HaCaT regarding UMP/CMPK1 downregulation. (C) Determination of CMP, CDP and CTP levels in parental and CDV^R cells by HPLC. Fold change is indicated when the difference in level of nucleotides between the parental and the CDV^R cell is statistically significant. (D) Ratio of parental over CDV^R cells CTP and UTP levels determined by means of HPLC following radioactive cytidine and uridine supplementation. Radiolabeled nucleosides where added to the cell medium and CTP and UTP levels were measured after 6 h and 24 h. No CTP level was detected in HaCaT_CDV cells, preventing the comparison to the parental cells.