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. 2016 Feb 6;7(9):10402–10413. doi: 10.18632/oncotarget.7224

Figure 3. Knockdown of OLA1 results in attenuation of TGF-β1-induced EMT in A549 cells.

Figure 3

A. A549 Cells were transfected with OLA1 siRNA (siOLA1) and control siRNA (siControl) for 48 hours, and then incubated with 2.5 ng/mL TGF-β1 or without TGF-β1 (−) in DMEM containing 5% FBS for 72 hours. Cell images were taken under an inverted light microscope (200×original magnification). B. Cell lysates were subjected to Western blot analysis with antibodies as indicated. β-actin was used as a loading control. C. Densitometric analysis of Western blots obtained in B., showing band intensities of OLA1 and E-cadherin normalized by that of β-actin. Data represent mean ± SD values from 3 independent experiments. * p < 0.01, as compared with the siControl group without the treatment of TGF-β1.Δp < 0.01, as compared with the Control siRNA group with or without the treatment with TGF-β1.