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. 2016 Feb 7;7(9):10459–10471. doi: 10.18632/oncotarget.7227

Figure 2. Targeting HO-1 by SnPP inducing KSHV-infected endothelial cell death is independent of apoptosis.

Figure 2

A. TIVE-LTC and TIVE were incubated with indicated concentrations of SnPP for 48 h, then cell proliferation was measured by the WST-1 assays as described in the Methods. B.-C. TIVE-LTC and TIVE were incubated with vehicle or indicated concentrations of SnPP for 24 h, then cell viability and apoptosis were measured by Annexin V-PI staining and flow cytometry analysis. TIVE-LTC were shown as an example for cell subpopulation diagram in panel C. D.-E. TIVE-LTC were incubated with vehicle or 25 μM of SnPP for indicated time, then cell viability and apoptosis were measured as above. F. TIVE-LTC were incubated with or without the pan-caspase inhibitor Z-VAD-FMK (ZVAD, 25 μM) for 2 h, followed by vehicle or 25 μM of SnPP treatment for another 2 h. Error bars represent the S.E.M. for 3 independent experiments. * = p < 0.05, ** = p < 0.01.