Fig. 3. Comparison of dynamic NF-κB binding patterns between the ethanol and carbohydrate control groups.

(A) COMPACT matrix showing the number of NF-κB binding targets for each combination of dynamic binding patterns between the ethanol and carbohydrate groups. The element at the i^th row and j^th column of the COMPACT matrix contains the number of genes that show i^th NF-κB binding pattern in carbohydrate group and j^th NF-κB binding pattern in ethanol group. The diagonal of the matrix represents those genes showing a common NF-κB binding response and the off-diagonal elements represent the genes showing an altered temporal NF-κB binding response between the dietary groups. The matrix was partitioned such that the patterns representing “no NF-κB binding” and “baseline NF-κB binding” separate the NF-κB binding in response to PHx. (B) A chord diagram visualization of the COMPACT matrix in A. The length of the arcs is proportional to the number of genes showing the corresponding NF-κB binding pattern. The width of the ribbons connecting the arcs corresponds to the comparative pattern count, i.e., the number of genes showing the corresponding NF-κB binding patterns in the two dietary groups. The arcs are arranged such that the “no NF-κB binding” pattern separates the patterns with and without NF-κB binding at baseline (lower and upper segements, respectively). (C-F) Select patterns are highlighted in the chord diagram to illustrate the visual analysis approach – NF-κB binding occurring only at 6h post PHx (C), only 1hr post PHx (D), at baseline (E), and at baseline and 1hr post PHx (F).