Table 1.
Analysis of dn-cHSF1-mediated heat shock response inhibition specificity (see also Table S1).
| RNA Array Fold Change Relative to Vehicle-Treated Cellsa | ||||
|---|---|---|---|---|
|
| ||||
| Gene | Arsenite | Arsenite + dn-cHSF1 | HSP90 Inhibitor | HSP90 Inhibitor + dn-cHSF1 |
| Cytosolic Chaperones | ||||
|
| ||||
| HSPA1A; HSPA1B | 13.15 | 0.52 | 18.68 | 1.19 |
| HSPA6; HSPA7 | 18.04 | 0.66 | 8.47 | 0.62 |
| HSPA6 | 13.88 | 0.85 | 7.04 | 0.72 |
| DNAJB1 | 3.43 | 0.38 | 3.31 | 0.38 |
| HSPH1 | 2.28 | 1.29 | 2.92 | 0.95 |
| DNAJA1 | 1.76 | 0.94 | 1.82 | 0.9 |
| HSP90AA1 | 1.39 | 0.66 | 1.78 | 0.69 |
|
| ||||
| Oxidative Stress Genes | ||||
|
| ||||
| HMOX1 | 27.67 | 16.92 | 1.15 | 0.8 |
| CTH | 8.47 | 8.37 | 1.3 | 3.47 |
| MT1X | 25.55 | 24.8 | 1.16 | 1.33 |
| MT1F | 16.2 | 19.01 | 1.13 | 1.44 |
|
| ||||
| Zinc Metalloproteins and Transporters | ||||
|
| ||||
| ZNF195 | 2.09 | 2.29 | 1.22 | 1.34 |
| ZNF33A | 1.4 | 1.33 | 1.12 | 1.01 |
| ZFP69B | 5.65 | 6.93 | 1.09 | 1.8 |
| ZNF10 | 2.19 | 2.13 | 1.07 | 1.16 |
| SLC30A1 | 3.37 | 4.05 | 1.06 | 0.97 |
| ZMYM3 | 0.45 | 0.43 | 1.06 | 0.85 |
| DPF2 | 1.51 | 1.52 | 0.98 | 0.98 |
| SLC30A2 | 6.00 | 12.89 | 0.96 | 1.3 |
| SLC30A1 | 4.34 | 4.92 | 0.92 | 0.98 |
| ZNF674 | 2.33 | 2.21 | 0.73 | 1.07 |
|
| ||||
| Endoplasmic Reticulum Chaperones | ||||
|
| ||||
| HSPA5 (GRP78/BIP) | 0.81 | 0.83 | 0.77 | 0.96 |
| GRP94 | 0.88 | 0.84 | 0.98 | 1.01 |
| DNAJB11 (ERDJ3) | 0.81 | 0.89 | 0.85 | 0.81 |
| DNAJB9 (ERDJ4) | 1.06 | 0.99 | 1.00 | 1.22 |
| DNAJC10 (ERDJ5) | 1.01 | 0.80 | 0.99 | 0.93 |
| HYOU1 | 1.04 | 0.98 | 1.13 | 1.05 |
|
| ||||
| Disulfide Redox | ||||
|
| ||||
| PDIA3 | 1.15 | 1.00 | 1.01 | 1.08 |
| PDIA4 | 1.04 | 1.00 | 1.15 | 1.17 |
| PDIA6 | 0.85 | 0.75 | 0.90 | 0.99 |
| ERO1L | 0.93 | 0.83 | 0.97 | 1.02 |
| ERO1LB | 1.11 | 1.24 | 0.73 | 1.03 |
a
Bolded text indicates an ANOVA p-value <0.001 relative to vehicle-treated cells. All un-bolded text indicates no significant change with respect to vehicle-treated cells.